‘My Swordhand is Singing’ Develops Essay

This tale is based around a little town called Chust, in Romania. Chust is a little seventeenth century town encompassed by an enormous and vile wood, loaded with shadows of anxiety. The residents were scared of the wood, and all that it contained. They were careful about ‘outsiders’ and were driven by convention. They particularly had confidence in the Miorita, a melody about a shepherd killed by his colleagues and through a sheep, he reaches out to his mom that he isn't dead yet has gone to ‘marry a princess from a far off land.’ They trusted in vampires and demons and the Shadow Queen. Dwindle and Tomas carried on with an itinerant life. They moved all around and were not acknowledged in Chust. They were wood-cutters and lived on a little island with streaming water around it. Diminish doesn't comprehend the hugeness of this canal and thinks it is only an endeavor to attempt to bond with him. It was the first run through in quite a while where Peter and Tomas were cooperating and appreciating it. It is their very own representation presence; desolate, separated and cryptic. Tomas has no enthusiasm for making companions and avoids society. This implies Peter doesn’t get the opportunity to make connections as they are continually moving near. This intensifies his feeling of disconnection and causes him to dislike his dad more. Dwindle doesn't comprehend what Tomas was fleeing from, intellectually or genuinely. Before Peter was conceived, Tomas was selected to battle with King Michael, the ‘Winter King’. The battling was long and savage and there was heaps of slaughter. While attacking a little Turkish town, Peter found a blade. This was an extraordinary blade. It had the ability to slaughter vampires. â€Å"The blade was made in a land were vampires were normal. This blade has the ability to vrykolakoi for good.† Vrykolakoi are the living dead, spirits, getting away from carcasses. When Tomas’s spouse kicked the bucket bringing forth Peter, Tomas accepted his life wasn’t worth living. He had lost the main individual who had thought about him, who adored him. This was unexpected in light of the fact that his activity was to return dead spirits who need to be alive and here he is with no will to live and needing to pass on. He doesn’t perceive the necessities of the living as he does the need of the dead. Tomas begins drinking to numb the agony of his wife’s demise. This is one of the fundamental driver of Peter and Tomas having a discontinuous relationship. Tomas gets goaded as Peter consistently attempts to stop him drinking yet Tomas considers it to be an intrusion of freewill. â€Å"By the time Peter got inside, Tomas had just presented himself with a glass of rakia. ‘Have some?’ he inquired. Diminish shook his head. ‘For God’s sake!’ his dad yelled, all of a sudden. ‘For God’s purpose, drink with me for once!† Diminish is terrified of Tomas. He is scared by his dad. He feels that Tomas is considering him answerable for his wife’s demise. The live in their own universes. â€Å"Who is ‘we’? There is no ‘we’ here.† At the point when Peter was 5, Tomas had made him a wooden goose. Tomas was exceptionally talented at carpentry and it was a wonderful magnum opus, an endowment of adoration. Angrily, Tomas demolished the goose. By doing this he was breaking any bond that he had made with Peter. Diminish attempted to make another goose, however it was never as acceptable. This represents their relationship, never to be on a par with it used to be. As Peter was growing up, Tomas deceived him to shield reality from coming out about his past. At whatever point Peter went into Chust to convey logs, he would return with stories. Tales about groups of sheep being assaulted and individuals dieing. Dwindle would tell Tomas of these however he would be hateful of them, and me for trusting them. â€Å"There’s a wide range of upheaval in the village’, Peter said. ‘Sheep have been assaulted in their sheds.’ ‘So the wolves are getting eager. What of it?’ ‘It’s not wolves. Well that’s what they’re saying in Chust’ ‘Pah!’ Tomas spat on the floor. ‘Idiots! Furthermore, you’re and blockhead too for listening†. For whatever length of time that Peter could recollect, Tomas had a container. Tomas had stated, ‘Do not look in the container under any circumstances’. For Peter, this is simply one more riddle about his dad and impedes their relationship. Toward the finish of this novel, Tomas rode into the horde of prisoners and began killing them to the ground. Tomas realized he was going to pass on and this was a last exertion to show Peter that he generally adored him and that he was a decent dad. As soon Tomas was overwhelmed by the vampires, Peter quickly got the blade and battled until they were all dead. This is know as the ‘Elizabethan Revenge Tragedy’ expressing that is you’re father bites the dust you should retaliate for his demise. At the point when Peter returned from the fight he found a wooden goose in the apparatus shed. This was to represent that he has moved here and there for his entire life and he should continue moving, similar to a feathered creature. â€Å"Tomas’ eyes were shut, however in his psyche he could see Peter turning and stroking the cutting edge from side to side. ‘That’s it. Feel it.’ In his heart, he heard Peter’s answer. ‘Yes, Father. My swordhand is singing.’

SCM PepsiCo Essay Example for Free

SCM PepsiCo Essay The Beverage Industry is a full grown segment and incorporates organizations that advertise nonalcoholic and alcoholic things. Since development openings are not many contrasted with existing business, numerous individuals from the business attempt to enhance their contributions to all the more likely contend and addition share. As well, they may seek after rewarding conveyance game plans and additionally acquisitions to grow their activities, item portfolios, and geographic reach. Most values in this gathering are appropriate for moderate financial specialists. The biggest organizations offer dependable profits, with normal increments, or more normal Stock Price Stability. There are a couple of determinations for those that are increasingly bold. Such issues may serve a specific market specialty, for instance, caffeinated beverages or growing abroad markets. For the most part, the gathering turns in a consistent exhibition all through the business cycle, yet it will by and large endure in the most unpleasant of financial occasions PepsiCo is one of the biggest food and refreshment organizations on the planet. Its items incorporate an assortment of salty, sweet, and grain-based tidbits just as Csds and non-Csds. the organization is liable for the assembling, promoting, and deals of these products. It has 18 brands in its portfolio and is headquartered in New York. PepsiCo has two divisions: bottle sodas and juice fragment. In India the utilization extent of the two divisions are acceptable as contrast with the significant contender. PepsiCo have put around 12000 crore in India and has the a large portion of the piece of the pie in India in drinks industry. Mr. Anil R the Senior Manager of the composed exchange handles the significant zone of Bangalore. His jobs are to see the best possible working in composed exchange and to bring new business. OT has significant territory like cafés, bars, Cineplex diversion, visitor region, and other significant zone of people’s needs. He was baffled by the business disappointment which done by PSR. Mr. Anil R, has seen in the shocked visit of 20 outlets over the Bangalore is that the administrative of the pre deals official isn't ordinary and they are not taking up the request from the clients time to time, not posting up the grievance of the client which they are confronting or more that they are not getting together the objective which they are proposed. Additionally what Anil found that the VISI cooler is likewise abused by the costumer, they put various stuffs in the costumer with other organization refreshments bottle. Anil found that these little looking issues have a major disappointment in future since it looses the costumer as well as the income will diminish and for this he got the opportunity to answer his most noteworthy position. Taking the whole serious issue on account and to the thought Anil has begun the preparation procedure and genuinely necessary to his PSR Mr. Prajapati and to Mr. Shail Sharma. The 7 days preparing comprises of relational abilities, conduct aptitudes about the item with clear detail and other significant properties in regards to consumer loyalty. Anil just accentuations on the consumer loyalty, he not just taught his PSR about the administrative visits to take the request yet additionally he educated about the PepsiCo duty towards the costumer and about VISI cooler he likewise trained. For all these he has given stick guidance for his PSR to catch up the great work whenever got disappointment may lose the employment. After accepting the activity, Anil does a Market look into and thought about the report on client response. He found that Prajapati was well over the normal for its costumer. As it were, Prajapati keep up the great relations with its costumer. On the other Shail stills falling behind the exhibition. Anil’s end is that Prajapati is performing great over the normal level yet stills it’s not the up to the company’s perspective. Shail still face the disappointment. He neglects to welcome the significance of relationship. At his first deals meeting as ranking director Anil went over the money related information that exhibited the amount more costly is to gain new costumers than its present ones yet we are losing even our current once. Prajapati has 2 years of work involvement with PepsiCo while Shail has 4 years of involvement with the PepsiCo as PSR. Questions: 1 What strategy should Anil follow with respect to PSR working Condition? 2 Should he recruit new PSR? 3 Do you figure Anil accomplishing a decent work to give preparing program?

Cognitive Distortions and Stress

Cognitive Distortions and Stress Stress Management Print Cognitive Distortions and Stress By Elizabeth Scott, MS twitter Elizabeth Scott, MS, is a wellness coach specializing in stress management and quality of life, and the author of 8 Keys to Stress Management. Learn about our editorial policy Elizabeth Scott, MS Updated on January 03, 2020 Jamie Grill / The Image Bank / Getty Images More in Stress Management Effects on Health Management Techniques Situational Stress Job Stress Household Stress Relationship Stress When you think about your life, it is quite possible that your mind is playing tricks on you that can distort your view. Cognitive distortionsâ€"where your mind puts a ‘spin’ on the events you see  and attaches a not-so-objective interpretation to what you experienceâ€"happen all the time. They are especially common in people with depression and other mood disorders. Psychologist Aaron T. Beck originally came up with the theory of cognitive distortions in the 1960s, and many therapists since then have helped clients live more positive lives by hunting down their cognitive distortions and correcting them. (It’s one of the tenets of a very successful and fast-working mode of therapy called cognitive therapy.) When you know what to be on the lookout for, it becomes rather easy to spot the cognitive distortions in others. It may be a little more challenging to spot your own, but it is possible. Doing so usually brings lasting positive change in the way you experience stressors in your life. An interesting thing to note is that several cognitive distortions  can actually work to your advantage. The key is to know when and how to do so. Here are the 10 most common (and officially recognized) cognitive distortions, with examples of how they relate to stress. You might find yourself smiling as you recognize one or two as familiar friends. If in the coming days, you look for them and gently correct them, you’ll be well on your way to reducing your reactivity to the stress in your life. All-or-Nothing Thinking This type of distortion is the culprit when people think in extremes, with no gray areas or middle ground. All-or-nothing thinkers often use words like always and never when describing things. “I always get stuck in traffic!” “My bosses never listen to me!” This type of thinking can magnify the stressors in your life, making them seem like bigger problems than they may, in reality, be. Overgeneralization Those prone to overgeneralization tend to take isolated events and assume that all future events will be the same. For example, an overgeneralizer who faces a rude sales clerk may start believing that all sales clerks are rude and that shopping will always be a stressful experience. Mental Filter Those who tend toward mental filtering may gloss over positive events and hold a magnifying glass to the negative. Ten things can go right, but a person operating under the influence of a mental filter may only notice the one thing that goes wrong. (Add a little overgeneralization and all-or-nothing thinking to the equation, and you have a recipe for stress.) Disqualifying the Positive Similar to mental filtering, those who disqualify the positive tend to treat positive events like flukes, thereby clinging to a more negative worldview and set of low expectations for the future. Have you ever tried to help a friend solve a problem, only to have every solution you pose shot down with a Yeah but... response? You’ve witnessed this cognitive distortion firsthand. Jumping to Conclusions People do this one all the time. Rather than letting the evidence bring them to a logical conclusion, they set their sights on a conclusion (often negative)  and then look for evidence to back it up, ignoring evidence to the contrary. The kid who decides that everyone in his new class will hate him, and ‘knows’ that they’re only acting nice to him in order to avoid punishment, is jumping to conclusions. Conclusion-jumpers can often fall prey to mind reading (where they believe that they know the true intentions of others without talking to them) and fortune-telling (predicting how things will turn out in the future and believing these predictions to be true). Can you think of examples of adults you know who do this? I bet you can. Magnification and Minimization Similar to mental filtering and disqualifying the positive, this cognitive distortion involves placing a stronger emphasis on negative events and downplaying the positive ones. The customer service representative who only notices the complaints of customers and fails to notice positive interactions is a victim of magnification and minimization. Another form of this distortion is known as  catastrophizing, where one imagines and then expects the worst possible scenario. It can lead to a lot of stress. Emotional Reasoning This one is a close relative of jumping to conclusions in that it involves ignoring certain facts when drawing conclusions. Emotional reasoners will consider their emotions about a situation as evidence rather than objectively looking at the facts. “I’m feeling completely overwhelmed, therefore, my problems must be completely beyond my ability to solve them,” or, “I’m angry with you; therefore, you must be in the wrong here,” are both examples of faulty emotional reasoning. Acting on these beliefs as fact can, understandably, contribute to even more problems to solve. Should Statements Those who rely on ‘should statements’ tend to have rigid rules, set by themselves or others, that always need to be followed â€" at least in their minds. They don’t see flexibility in different circumstances, and they put themselves under considerable stress trying to live up to these self-imposed expectations. If your internal dialogue involves a large number of ‘shoulds,’ you may be under the influence of this cognitive distortion. Labeling and Mislabeling Those who label or mislabel will habitually place labels that are often inaccurate or negative on themselves and others. “He’s a whiner.” “She’s a phony.” “I’m just a useless worrier.” These labels tend to define people and contribute to a one-dimensional view of them, paving the way for overgeneralizations to move in. Labeling cages people into roles that don’t always apply and prevents us from seeing people (ourselves included) as we really are. It’s also a big no-no in  relationship conflicts. Personalization Those who personalize their stressors tend to blame themselves or others for things over which they have no control,  creating stress  where it need not be. Those prone to personalization tend to blame themselves for the actions of others  or blame others for their own feelings. If any of these feel a little too familiar, that’s a good thing: recognizing a cognitive distortion is the first step of moving past it.

The Effects Of Stress On The Body - 3325 Words

We are pleased to present you with our technical report, Is Merely Managing Our Stress Enough? This report addresses the topic of stress and how we manage it in our daily lives. We are hoping others will be influenced to consider looking at stress a new way. This report has enabled us to gain further knowledge and a fresh perspective on stress. Many different aspects regarding stress, its effects on the body, how to manage it, and new ways to think of stress are included. There are many different variables to consider regarding stress. Our research has allowed us to address some of these circumstances. Scientist Hans Selye defined stress in the 1930’s. His research is the foundation upon which stress information has been built.†¦show more content†¦We have put a sincere effort toward a presentable document, and making it as informative and articulate as possible. Our study involved three areas of interest. First, defining stress, considering the history and the basic physical process involved when faced with a stressor. Second, we looked at the current methods for managing stress and the positive impacts of such practices. Finally, we considered the newest research about how changing our perception of stress and our perceived control of stress could benefit us with healthier and longer lives. We learned that our body s reaction to stress is biological and automatic and has been a function of our survival for as long as mankind has existed. It is what kept our ancestors alive. Today, it can be what kills us. Current methods for handling stress include managing our body, managing our situation and managing our mind. Managing our body has to do with trying to regulate our response to stressors with practices like meditation, relaxation, and exercise. Managing our situation involves our social support through relationships with our loved ones and friends. It can include religious groups we might belong to or a practice of spirituality that enables us to deal with the stress in our lives. Managing our mind is done through coping mechanisms, some of which are automatic and

The Truth Behind Alzheimers Disease - Free Essay Example

Sample details Pages: 6 Words: 1902 Downloads: 3 Date added: 2019/04/12 Category Medicine Essay Level High school Tags: Alzheimer's Disease Essay Did you like this example? Its not uncommon for an individual to suffer from episodes of forgetfulness, it happens everyday, in fact it is partly a good thing that we forget certain things because if we didnt, our minds would be over filled with all the information we have ever retained. However the things we tend to forget are usually small and wont affect our lives to drastically. For example, forgetting the name of a song you recently learned about or my favorite, when men forget to put the toilet seat down. Don’t waste time! Our writers will create an original "The Truth Behind Alzheimers Disease" essay for you Create order But what would you do if your memory was so bad that you began to forget how complete simple everyday tasks, and couldnt ever remember how to do so. Imagine waking up in the morning and not knowing or understanding where you are. Or on the other hand, imagine looking at one of your parents and they fail to recognize you one day. Alzheimers disease is a brain disease in which damaged and dying brain cells cause mental deterioration over a period of time (Blackwell and Manar, 2015 p.125). The problem with alzheimers disease is that it is an irreversible disease that progresses over time and nearly anyone can get it! According to Kivipelto and Hakansson (2017), in the US, alone 32% of people over the age of 85 have been diagnosed with alzheimers and this is expected to increase. There is an estimated 50 million people with alzheimers disease and, By 2050 if no new treatments succeed in delaying the condition, more than 130 million may suffer with some form of the disease. About 60 to 70 percent of these patients will have Alzheimers dementia, and around 20 to 25 percent will be classified as suffering from the vascular form of the illness. (Kivipelto and Hakansson, 2017). Thats a scary thought to think about because this disease could affect, your parents, your spouse or more scarily yourself! I personally get to see my residents at work suffer from the disease which lead me to be more and more curious as to what it actually was. What causes Alzheimers, how does someone take care of one with the disease and what does one with this disease have to deal with in terms of behavior are all questions that came to mind after first hearing about the disease. We may never know why some people get alzheimers and other people do not but according to Bennett (2016), and although there is no known cause, genetics come into play and some people are just unfortunate to inherit high-risk genes associated with the disease. This disease deteriorates the brain so much that even the size of the brain shrinks according to Blackwell and Manar, (2015). This is because with alzheimers disease follows brain cell death. The cause of this cell death is greatly explained as follows: Today we know that these classic features are accumulations of malfunctioning proteins†mostly misfolded fragments of beta-amyloid in the plaques and abnormal tau in the tangles (Bennett, 2016) It is normal for any brain that has gone through the natural process of life through late adulthood to exhibit some plaque and/or tangles in the brain, however a brain diagnosed with alzheimers has relatively more. According to research you are more likely to get alzheimers if you haveadvanced age, trauma such as head injury, and gene mutations (Blackwell and Manar, 2015 p.127). There is no known treatment to cure or prevent the progression however researchers have been trying and trying for years. Not only are there over 100 ongoing clinical trials to create a drug for alzheimers, but also More than 200 experimental drugs intended to treat it have failed during the past 30 years. (Kivipelto and Hakansson, 2017). Throughout the years research has come up with some theories that have been proven to help prevent the onset of alzheimers. For example maintaining good cardiovascular health, a well-balanced diet, the achievement of higher education levels and having an active social life all diminish the risk of getting the disease as stated by Kivipelto and Hakansson, (2017). Research suggests that a good specific diet that could dramatically lower the risk of developing alzheimers is the MIND diet which is known to be rich in berries, vegetables, whole grains a nd nuts (Bennett, 2016). In the brief time of my CNA career I watched a very close resident of mine decline drastically and eventually die of Alzheimers. When I first met her, she came to know and remember who I was very quickly but closer to the time of her passing, it was clear that she did not remember my name and wasnt ever going to. In her final stages, she seemed like an entirely different person. After doing research I learned that some of the behaviors and symptoms accompanied with this disease include inattention to personal hygiene, forgetfulness, lack of concentration and impaired judgment with later symptoms being the inability to read, walk, speak, take care of themselves and remember their loved ones (Blackwell and Manar, 2015 p.125). Learning this helped me understand why my dear friend would often not make any sense when she was talking and do stuff out of the ordinary like walk around in the middle of the night collecting things. I would see her agitated, wandering and repeating the things she did. Her behaviors were much worse when I worked the evening shift, which I came to learn was associated with sundowning, a common side effect many Alzheimers victims have to deal with, like Kalb, Wingert, Grossman, Weingarten, and Raymond, (2000) stated, For some unknown reason, people with Alzheimers can become increasingly agitated at the end of the day. I was taken by surprise to see how often these people become combative also. We may just be providing help to this person, but in the eyes of someone who has the disease they may not understand what you are doing or think you are hurting them, so they proceed to being combative to protect themselves. Hitting, scratching, biting, and kicking are all very common acts that someone with Alzheimers disease may display. Caring for a loved one with Alzheimers is a horrific burden for most peoplestressful and enormously expensive, with few services covered by insurance (Kalb, Wingert, Grossman, Weingarten, and Raymond, 2000) . According to Kalb, Wingert, Grossman, Weingarten, and Raymond, (2000), 70 percent of people with alzheimers live at home and of every every for caregivers, three of them are women. These caregivers are ripped apart both emotionally by watching their loved ones deteriorate and suffer, but also financially as, $174,000 The average cost of caring for a person with Alzheimers throughout the course of the disease (Kalb, Wingert, Grossman, Weingarten, and Raymond, 2000). This often brings depression as studies show that one third these caregivers suffer from serious depression Kalb, Wingert, Grossman, Weingarten, and Raymond, (2000). Since the other 30 percent of alzheimers patients reside in nursing homes like the one I work at, I decided to turn to my nurses at my job who had more experience for answers. A particular nurse that had been working with alzheimers patients for nearly 25 years told me that caring for someone with Alzheimers is very difficult and every persons case is different. If you dont have patience you will not be able to take care of that person. You might have to answer the same question 100 times or more because within seconds of the conversation they have already forgot the question you asked or even asking the question. When caring for someone the with disease you might have to help them get dressed or dress them completely and feed them. If they reach the later stages of Alzheimers they may become totally dependent on others and for some families they are just not able to take care of another human being so they resort to nursing homes and other care facilities. Caring for those in that k ind of setting requires them to be locked inside of the unit because it is very dangerous for them to go out on their own into the outside world yet that is the goal for many of them who are trying to return to a place more comfortable to them. Something she told me that I found fascinating was that when she works with them she has to get into their world. In their brain they may think that they are in a different time period in their lives and be confused because they are somewhere other than where they are thinking. To successfully help that person you have to go along with what they are saying as you try and redirect them so that you dont upset them and make the situation worse. More attempts to enhance the care for alzheimers patients as, Researchers are also experimenting with ways to calm residents fiddling, pacing and outbursts, using everything from aromatherapy to aquariums (Kalb, Wingert, Grossman, Weingarten, and Raymond, 2000). In a recent study, simple one-on-one intera ctions helped reduce half the disruptive verbal outbursts in a nursing home. Nursing homes are also making advancements such as creating smaller, homier setting for the residents equipped with kitchens and laundry rooms to engage those who have lost the capacity to do other things on their own as well as keep them busy. Just last week my facility switched out all of our overhead lights for much brighter lights which corresponds with the research that, Specialists are calling for lots of light to eliminate frightening shadows and help reduce depression, which is also widespread in the Alzheimers population. Light is especially important in the late afternoon and early evening, when a phenomenon known as sundowning kicks in. (Kalb, Wingert, Grossman, Weingarten, and Raymond, 2000). Alzheimers is a terrible disease that can be compared to, losing pages from a chronological photo album of your life from back to front†with childhood memories the last to go (Bennett, 2016). There are many different foundations such as the Walk to End Alzheimers to help raise money for the research of this disease as well as caring for those with it. I am excited to be able to better help my residents and expand my understanding about a disease that is so common in the United States especially since baby boomers are reaching late adulthood. It hurts my heart to know that millions of people have to suffer from this disease, however now knowing the signs helps me to be less fearful for the future because I can better handle a situation if I or a loved one ever develops the disease. I encourage everyone else to become more knowledgeable of the signs since the population of those who have it is continuously increasing and it does not seem to be going away anytime soon. With this new information we should all take measures to live a healthy lifestyle and hopefully avoid getting alzheimers. Reference Alzheimers Disease. (2015). In A. H. Blackwell E. Manar (Eds.), UXL Encyclopedia of Science (3rd ed., Vol. 1, pp. 125-128). Farmington Hills, MI: UXL. Retrieved from https://frccwc.idm.oclc.org/login?url=https://go.galegroup.com.frccwc.idm.oclc.org/ps/i.do?p=GVRLsw=wu=west59484v=2.1it=rid=GALE%7CCX3618300040asid=907cac0de484e31a17916394e5d8bd85 Bennett, D. A. (2016). BANKING AGAINST ALZHEIMERS. (cover story). Scientific American Mind, 27(4), 28-37. Kalb, C., Wingert, P., Grossman, K. N., Weingarten, T., Raymond, J. (2000). Coping With the Darkness. (cover story). Newsweek, 135(5), 52. Kivipelto M, Hkansson K. A RARE SUCCESS AGAINST ALZHEIMERS. (cover story). Scientific American [serial online]. April 2017;316(4):32-37. Available from: Psychology and Behavioral Sciences Collection, Ipswich, MA. Accessed April 28, 2017.

Human Embryonic Stem Cells Free Essays

Human embryonic stem (hES) cells have the unique capability of differentiating into all cell types, leading to the development of an entire organism. As the integrity of ES cells is critical for the developing embryo, these cells have likely evolved mechanisms that detect and respond rapidly to adverse stimuli. Indeed, hES cells have been shown to be highly sensitive to DNA damage, but the molecular mechanisms underlying this rapid death remain unclear. We will write a custom essay sample on Human Embryonic Stem Cells or any similar topic only for you Order Now Caspases are critical mediators of apoptosis in mammalian cells, and a key protein that controls their activation is Bax, a proapoptotic member of the Bcl-2 family. While the main components of the apoptotic pathway have been identified, exactly how this pathway is regulated in various primary cells remains unclear. Here, we examined the apoptotic pathway in hES cells and report a unique mechanism engaged by hES cells that can prime them to undergo rapid apoptosis inresponse to genotoxic damage.To visualize GFP-tagged Bax, 3-day colonies of hES cells were transfected with 2 mg of hBaxC3-EGFP (Addgene) with FuGENE HD transfection reagent. The process of introducing nucleic acids into eukaryotic cells by nonviral methods is defined as transfection. Using various chemical, lipid or physical methods, this gene transfer technology is a powerful tool to study gene function and protein expression in the context of a cell. Development of reporter gene systems and selection methods for stable maintenance and expression of transferred DNA have greatly expanded the applications for transfection. Assay-based reporter technology, together with the availability of transfection reagents, provides the foundation to study mammalian promoter and enhancer sequences, trans-acting proteins such as transcription factors, mRNA processing, protein:protein interactions, translation and recombination events (Groskreutz and Schenborn, 1997). Transfection is a method that neutralizes or obviates the issue of introducing negatively charged molecules (e.g., phosphate backbones of DNA and RNA) into cells with a negatively charged membrane. Chemicals like calcium phosphate and DEAE-dextran or cationic lipid-based reagents coat the DNA, neutralizing or even creating an overall positive charge to the molecule. This makes it easier for the DNA:transfection reagent complex to cross the membrane, especially for lipids that have a â€Å"fusogenic† component, which enhances fusion with the lipid bilayer. Physical methods like microinjection or electroporation simply punch through the membrane and introduce DNA directly into the cytoplasm. Here we describe the striking observation that healthy undifferentiated hES cells maintain Bax in its preactivated state at the Golgi. This is in contrast to other cell types in which Bax is typically present in an inactive form in the cytosol. Our results also highlight the fact that the apoptotic machinery undergoes dynamic changes even at early stages of differentiation.While undifferentiated hES cells have constitutively active Bax and undergo rapid apoptosis in response to DNA damage, just 2 days of differentiation induced significant changes suchthat Bax was no longer active, and the cells were no longer highly sensitive to DNA damage. This could be manifested with even greater complexity in vivo as cells during early embryogenesis undergo rapid proliferation and differentiation. How to cite Human Embryonic Stem Cells, Papers

Artistic fashion Essay Example For Students

Artistic fashion Essay The main reason why such a large percentage of businesses in the modern world use Information Technology to promote their business is because it provides a totally professional look. The way a computer can present information, such as an advertising poster or an official letter to a customer or to a potential investor, is so much more professional and almost to perfection that no human hands could match it. When, in the instance of this task, a new business wishes to promote itself as a new, efficient, modern company, but also with the touch of professionalism required to be successful in the hotel industry, it is necessary to be able to promote yourself in this image, by using modern means of communication, such as the computer, and in this way, the IT user gains the advantage of a more professional look to the item that the non-IT user can simply not attain. Another disadvantage to the non-IT user is the question of the time it takes to mass-produce items. If 1000 copies of a flyer are required for a promotion of your company, and they must first be hand-designed, and then hand-written, it is going to take a very long time to complete. However, by designing the work on computer, that worker could have saved hours even days, by simply instructing the computer to print more copies, or maybe even using a photocopier to reproduce his work, as Information and Communication Technology is not just about computers, but also about the way humans interact with technology itself. However, the IT user can have many troubles in his quest for a perfect piece of IT work that are encountered simply because he is using IT. A computer may be infallible in that it only computes what it is fed, but there are numerous ways in which information can be corrupted so that it is no longer useable. For instance, viruses are a major problem for computer users, especially those who are connected to the Internet. The virus can destroy the information stored on the computer totally; even destroy the computer itself. More about this has been written in a later section of this project, which relates directly to the production of my task, but it is obvious that this is not a problem the non-IT user is going to encounter. For this task, I found that the use of IT provided me with many advantages, but it also proved to be a hindrance in other ways. The table below will outline these factors. Advantages Disadvantages I made many errors during the course of my work, but using a computer meant that I did not have to start again. The system I used was easy to corrupt, especially with so many other users of the server, and the computers did crash and lose some of my work numerous times. I had to produce my work twice. By using it does not require you to copy the whole piece of work again. Computers are susceptible to viruses, and I was lucky at one point not to lose my entire computer to a virus someone attempted to send me, which could have destroyed my machine as well as my work. Advantages Disadvantages I was able to give my work a more professional look that would have been impossible to produce without a computer I found that using computer aided design for this project was sometimes limiting in the amount of freedom I had to design things in an artistic fashion, whereas if I had done my designs freehand, I would not have had as many limitations Copyright There are many laws governing the misuse of data that is copyright protected. Copyright is a protection that covers published and unpublished literary, scientific and artistic works, whatever the forms of expression, provided such works are fixed in a tangible or material form. .uadb414ff3a6c8973596ff118cf5288fc , .uadb414ff3a6c8973596ff118cf5288fc .postImageUrl , .uadb414ff3a6c8973596ff118cf5288fc .centered-text-area { min-height: 80px; position: relative; } .uadb414ff3a6c8973596ff118cf5288fc , .uadb414ff3a6c8973596ff118cf5288fc:hover , .uadb414ff3a6c8973596ff118cf5288fc:visited , .uadb414ff3a6c8973596ff118cf5288fc:active { border:0!important; } .uadb414ff3a6c8973596ff118cf5288fc .clearfix:after { content: ""; display: table; clear: both; } .uadb414ff3a6c8973596ff118cf5288fc { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .uadb414ff3a6c8973596ff118cf5288fc:active , .uadb414ff3a6c8973596ff118cf5288fc:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .uadb414ff3a6c8973596ff118cf5288fc .centered-text-area { width: 100%; position: relative ; } .uadb414ff3a6c8973596ff118cf5288fc .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .uadb414ff3a6c8973596ff118cf5288fc .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .uadb414ff3a6c8973596ff118cf5288fc .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .uadb414ff3a6c8973596ff118cf5288fc:hover .ctaButton { background-color: #34495E!important; } .uadb414ff3a6c8973596ff118cf5288fc .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .uadb414ff3a6c8973596ff118cf5288fc .uadb414ff3a6c8973596ff118cf5288fc-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .uadb414ff3a6c8973596ff118cf5288fc:after { content: ""; display: block; clear: both; } READ: 1950s mens fashion EssayThis means that if you can see it, hear it or touch it it may be protected. If it is an essay, a play, a song, a photograph, HTML coding or a computer graphic that can be set on paper, recorded on tape or saved to a hard drive, it may be protected. Copyright laws grant the creator the exclusive right to reproduce, prepare derivative works, distribute, perform and display the work publicly. Exclusive means only the creator of such work, not anybody who has access to it and decides to grab it. These laws mean that I am not allowed to copy anybody elses work, nor give out any private details. I have been sure to that I have not used anybody elses designs in my work, and I have asked the permission of the companies involved in my research. They are acknowledged here: Company Acknowledgement Marriott Hotels For the information pack they sent me for my research Greg Barker of Simrad Ltd. For the use of his personal business card Fortek Computers Ltd. For the use of their letterhead design Error Handling and Virus Protection I was fortunate to avoid most of the errors that could easily have been encountered in my coursework. For instance, I did not have any problems with files corrupting themselves, or any attacks of viruses. I did come across a few problems with file sharing violations when an unnamed individual also working on this project discovered my server access password and attempted to change my files. However, this problem was quickly averted from reoccurring by password protecting my files and changing my user account password. I made numerous spelling mistakes in the course of this project, which I was able to avert with the use of a spelling checker, and then proof reading the work. I also asked my father to read the work, and he was able to help me correct a few spelling mistakes. It was necessary for me to password protect my files during the course of the project, to avoid attacks from other users. I also decided to backup my files on my own floppy disk, so that I could retrieve my files even if they were destroyed for some reason. A screen print of my spell check. My floppy disk for backing up files. Other errors I encountered included a faulty printer, which turned out to have no ink left, and also the program I was using telling me not to overwrite a file that I was currently accessing. When using computers, it is vital to ensure that you use correct posture, to avoid giving yourself any unnecessary aches and pains because you sit incorrectly. You should take a break from computers every hour for at least 15 minutes, to avoid becoming transfixed by the computer screen and causing problems with your eyes, and to avoid over-exposure to computer radiation. Computer users should never turn a computer off while they are still working on it, by pressing emergency cut off switches, as it can damage the computers and also blow the fuses running them. Computer users must be wary of viruses, especially when computers are connected to the Internet. It is very simple to do, as you must simply run a virus-checking program, such as Norton AntiVirus, which highlights any viruses, and have it constantly checking any downloaded files for errors.